Blood is drawn from a vein, usually from the inside of the elbow or the back of the hand. The site is cleaned with germ-killing medicine (antiseptic). The health care provider wraps an elastic band around the upper arm to apply pressure to the area and make the vein swell with blood.
Next, the health care provider gently inserts a needle into the vein. The blood collects into an airtight vial or tube attached to the needle. The elastic band is removed from your arm.
Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.
In infants or young children, a sharp tool called a lancet may be used to puncture the skin and make it bleed. The blood collects into a small glass tube called a pipette, or onto a slide or test strip. A bandage may be placed over the area if there is any bleeding.
No special preparation is necessary for this test.
When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.
In some situations, your health care provider may check your antibody titer to see if you had an infection in the past (for example, chickenpox) or to decide which immunizations you need.
The antibody titer is also used to determine:
- The strength of an immune response to the body's own tissue in diseases such as systemic lupus erythematosus (SLE) and other autoimmune disorders
- Your need for a booster immunization
- Whether a recent vaccine caused a strong enough response from your immune system to protect you against the specific disease
- Whether you have, or recently had, an infection such as mononucleosis or viral hepatitis
Normal values depend on the antibody being tested. If your health care provider is testing for antibodies against your own tissue, then the normal value would be zero or negative. In some cases, a normal level is below a certain, specific number.
If your health care provider is testing to see if an immunization brought your antibody titer up to a preventive level, then the normal result depends on the specific value for that immunization.
Negative antibody tests can help rule out certain infections.
Normal value ranges may vary slightly among different laboratories. Talk to your doctor about the meaning of your specific test results.
If your health care provider is testing for antibodies against your own tissue, abnormal results would show a positive antibody titer. Depending on the strength of the titer, this could mean that you have an autoimmune disease in which your immune system is fighting its own tissue, cells, or substances.
If your health care provider is testing to see if your immunization brought your antibody titer up to a preventive level, an abnormal result would indicate that your body has not mounted enough of a response against the immunization and you are not fully protected against the disease.
A positive antibody test to infectious agents such as viruses can determine if you have a specific infection.
Low levels may also occur if you have an immune deficiency.
Veins vary in size from one patient to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.
Risks associated with having blood drawn are slight but may include:
- Excessive bleeding
Fainting or feeling light-headed
- Hematoma (blood accumulating under the skin)
- Infection (a slight risk any time the skin is broken)
Orenstein WA. Immunization. In: Goldman L, Ausiello D, eds. Cecil Medicine. 23rd ed. Philadelphia, Pa: Saunders Elsevier;2007:chap 16.
Pisetsky DS. Laboratory testing in the rheumatic diseases. In: Goldman L, Ausiello D, eds. Cecil Medicine. 23rd ed. Philadelphia, Pa: Saunders Elsevier;2007:chap 278.